Obstacles

Good Morning Everyone!

For the past few days in the lab, we have reached an obstacle in our research. As stated in my previous post, I have cast several gels and have run multiple PCRs. But recently, the gels have not been producing efficient results.

The confusion lies in why. Earlier last week, when I followed the original A140V PCR Protocol and ran the original "Main: A140V-HF Program" (the program that is running on the PCR machine), two gels worked. Now, when I am following that same protocol, I am not reaching conclusive results. My mentor and I have altered the protocol and even the program slightly each day in hopes to find the genotype of the samples.

Today, I will be following the original protocol one more time with a slightly altered PCR program. Fingers crossed for it to work!

Pooja